Relationship Between Maintaining Diet Containing High Fructose and Development of Polycystic Ovary Via Perilipin Expression

INTRODUCTION: Studies showing diet consisting of PCOS combined with high fructose are in a limited level. In this study, it is aimed to investigate PLIN2 and PLIN3 expressions by showing diet consisting of combination of high fructose and PCOS.
METHODS: A total of 26 Balb/C type female mice have been used and 3 groups are created: Control (no treatment), HF (tap water containing 20% fructose is given by oral gavege) and Hormone (The injection of DHEA is injected s.c.). In order to show the morphological differences between the groups haematoxylin and eosin staining method has been used. For PLIN2 and PLIN3 antibodies western blot method has been used. ELISA technic has been used to compare progesterone and LH levels between the groups.
RESULTS: It is seen that antral and preantral follicle numbers of HF and Hormone groups have increased. It is also seen that PLIN2 and PLIN3 expressions are high in HF and Hormone groups.
DISCUSSION AND CONCLUSION: As a result diet with high fructose could contribute to PCOS development and in this mechanism PLIN2 and PLIN3 proteins could also be effective.